Methods in the lab

Electrophysiology

  • patch-clamp
  • Ussing chamber (cell culture, freshly isolated intestinal tissues)
  • Xenopus oocyte TEVC
  • In vitro perfusion of renal tubules
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    Fluorescence imaging

  • [pH]i

    • [Ca2+]

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In vivo:

  • Metabolic analysis

  • Renal clearance measurements

  • Blood pressure measurements

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Molecular biology:

  • RNA, DNA preparation and analysis

  • Basic protein analysis, Western blotting

  • Immunohistochemistry

 

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